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pVY3
pVY3
規(guī)格:
貨期:
編號:B214000
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pVY3
商品貨號 B214000
Designations pVY3
GenBank Number

U08629

Species Yamadazyma stipitis (Pignal) Billon-Grand, teleomorph
Depositors TW Jeffries
Vector
Construct size (kb): 4.407999992370606
Insert
DNA: genomic
Insert lengths(kb): 1.445999979972839
Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3]
Insert Size (kb) 1.446
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SalI--4.8; SalI/XbaI--3.2, 1.6; XbaI--4.8.
The insert contains the following restriction sites (approximate kb from the 5' end): AccI--0.00, 0.62, 0.78; HindIII--0.16; KpnI--0.51; EcoRI--1.20.
References

Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063

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