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Construct size (kb): 48.0

DNA: genomic

Insert lengths(kb): 40.0

Gene product: DNA Segment, single copy [D5S81]

Alleles: A1, A2

Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 8.3, 7.0, 2.3; EcoRI--20.0, 8.5 (doublet), 2.5, 2.3, 1.4, 0.98; HindIII--7.0, 6.0, 5.4, 4.2, 3.9, 3.3, 3.2, 2.5, 2.0, 1.8, 1.7, 1.6, 1.0, 0.98;

BglII--15.0, 7.2, 6.6, 6.0, 5.4, 3.2, 2.2, 1.6, 1.5, 1.4; SalI--26.0, 23.1, 2.7.

Codominant segregation demonstrated in 60 families.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Enzyme(s) not detecting polymorphism: PstI, MspI.