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Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office (2007). The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

These cells have been growth-arrested by irradiation with 12,000 rads.  The cells will begin to deteriorate 2 weeks after plating and may no longer support the growth of cells. We recommend that you do not keep the cells in culture for longer than 2 weeks.

It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 10⁶ cells/T75 in order to obtain a 100% confluent monolayer for stem cells growth. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported.

Moore KA, et al. Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs. Proc. Natl. Acad. Sci. USA 94: 4011-4016, 1997. PubMed: 9108096

Miller JS, et al. Single adult human CD34(+)/Lin-/CD38(-) progenitors give rise to natural killer cells, B-lineage cells, dendritic cells, and myeloid cells. Blood 93: 96-106, 1999. PubMed: 9864151

Moore KA, et al. In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood 89: 4337-4347, 1997. PubMed: 9192756

Hachney JA, et al. A molecular profile of a hematopoietic stem cell niche. Proc. Natl. Acad. Sci. USA 99: 13061-13066, 2002. PubMed: 12226475

Punzel M, et al. The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro. Blood 93: 3750-3756, 1999. PubMed: 10339481

Thiemann FT, et al. The murine stromal cell line AFT024 acts specifically on human CD34+CD38- progenitors to maintain primitive function and immunophenotype in vitro. Exp. Hematol. 26: 612-619, 1998. PubMed: 9657136

Lewis ID, et al. Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood 97: 3441-3449, 2001. PubMed: 11369635

Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.