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Distribution host: Escherichia coli

Size (kb): 7.5500001907348630

DESCRIPTION OF VECTOR:
Intact vector size: 7.550
Type of vector: plasmid
Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Construction: pSE1107 (pACT1)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 activation domain, ->
epitope tag: hemagglutinin (HA), ->
MCS: BglII/NdeI...BglII, ->
terminator: ADC1
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR
replicon: pMB1

Vector: pACT2 (plasmid)

Promoters: Promoter ADC1 (ADH)

Construction: pSE1107 (pACT1)

Marker(s):LEU2,ampR

Construct size (kb): 7.550000190734863

Features: marker(s): LEU2
marker(s): ampR
other: GAL4 activation domain
promoter: ADC1 (ADH)
replicon: 2 micron
replicon: pMB1
MCS: BglII/NdeI...BglII
terminator: ADC1
epitope tag: hemagglutinin (HA)

YE-type (episomal) shuttle vector

expression vector

vector component of two-hybrid screening system

vector for constructing cDNA libraries

Restriction digests of the clone give the following sizes (kb): EcoRI--7.6; BamHI--7.6; BglII--7.5.

Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 activation domain (aa 768-881) and a HA (hemagglutinin) epitope tag in frame with the activation domain.

Temperature: 37.0°C

component of:ATCC 87014