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pcJE-1
pcJE-1
規(guī)格:
貨期:
編號(hào):B222750
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pcJE-1
商品貨號(hào) B222750
Designations pcJE-1
GenBank Number

M19681

Species Mus musculus, mouse
Depositors CD Stiles
Applications
Frequently used as a molecular marker for response to cellular growth factors.
Has been used to identify the human homolog from WI-38 cells.
Vector
Construct size (kb): 3.5
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: 3T3 (clone A31) cell line
Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) [Scya2]
Insert Size (kb) 0.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 0.63; BamHI--uncut; consistent with deposited DNA.
Transcription from the SP6 promoter yields sense RNA.
JE is one of several (including KC, JB) competence genes which are induced with the addition of platelet-derived growth factor. Frequently used as a molecular marker for response to cellular growth factors.
Has been used to identify the human homolog from WI-38 cells.
Includes a single open reading frame that presumably encodes a protein of 148 aa, with a consensus sequence for N-glycosylation at aa 126. The 3' region contains an ATTTA sequence described for cytokines and mRNAs with rapid turnover.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Cochran BH, et al. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33: 939-947, 1983. PubMed: 6872001

Rollins BJ, et al. Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties. Proc. Natl. Acad. Sci. USA 85: 3738-3742, 1988. PubMed: 3287374

Timmers HT, et al. E1A products of adenoviruses reduce the expression of cellular proliferation-associated genes. Oncogene Res. 3: 67-76, 1988. PubMed: 2974528

Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477

Wilson SD, Dorf ME. Localization of five cytokine genes to chromosome 11. Mouse News Lett. 85: 14-15, 1989.

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