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HSE26.1
HSE26.1
規(guī)格:
貨期:
編號(hào):B230699
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HSE26.1
商品貨號(hào) B230699
Designations HSE26.1
GenBank Number

J04071

Species Homo sapiens, human
Vector
Construct size (kb): 3.799999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIBI24
Intact vector size: 2.878
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Polylinker sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Construction: pEMBL
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GZMB
Other gene symbols: CTLA1; CSPB; CCPI; CGL-1
Genomic copy number: unique
Gene name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated
serine esterase 1)
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14 q11.2
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: J04071
Insert lengths(kb): 0.8999999761581421
Tissue: T lymphocyte, activated
Gene product: cytotoxic serine protease B [CCPI]
Insert Size (kb) 0.900
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.05, 1.35, 0.66; EcoRI--3.15, 0.92; HindIII--3.2, 0.82; PstI--3.65, 0.39; PvuII--2.7, 0.76, 0.36, 0.20; SalI--3.95.
Contains the complete coding sequence (nt 17-760).
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.2, 0.35; ApaI--0.22; PstI--0.35; BglI--0.4; TaqI--0.56; HindIII--0.72.
Detects genomic restriction fragments of the following sizes (approximate kb): human, BamHI--12; human, BglII--14; human, BclI--10; human, EcoRV--18; human, TaqI--3.5, 2.5; human, PstI--4.0, 2.2; mouse, PstI--4.0.
References

Trapani JA, et al. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. USA 85: 6924-6928, 1988. PubMed: 3261871

Klein JL, et al. Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes. Genomics 5: 110-117, 1989. PubMed: 2788607

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