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Distribution host: Escherichia coli MC1066

Size (kb): 7.3769998550415040

DESCRIPTION OF VECTOR:
Intact vector size: 7.377
Type of vector: plasmid
Cloning sites: NotI SacI SmaI BamHI XbaI
Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI
Other unique sites: HindIII
Host range: Saccharomyces cerevisiae; Escherichia coli;
Schizosaccharomyces pombe
Features (with orientation and position when available):
restriction site: HindIII, 1-1
promoter for in vitro transcription: T3, <-, 11-30
promoter: lac, <-, 100-128
replicon: pMB1, 454-454
marker(s): ampR, <-, 1212-2072
promoter for in vitro transcription: T7, ->, 3132-3151
MCS: NotI...XbaI, ->, 3180-3214
replicon: 2 micron/LEU2-d, 3208-5903
marker(s): LEU2-d, ->, 4570-5664
marker(s): URA4 from S. pombe, <-, 6055-6849

Vector: YEpFAT2 (plasmid)

Promoters: Promoter for in vitro transcription T3

Marker(s):ampR,LEU2-d,ura4+

Construct size (kb): 7.376999855041504

Features: marker(s): LEU2-d
marker(s): URA4 from S. pombe
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: NotI...XbaI
restriction site: HindIII

M9 + leucine + tryptophan culture media recommended for DNA purification.

Restriction digests of the clone give the following sizes (kb): BamHI--7.2; SmaI--7.2; EcoRI--5.4, 2.03.

M9 + leucine + tryptophan culture media recommended for DNA purification.

One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.

Temperature: 37.0°C