亚洲精品无码成人片久久不卡,亚洲自偷自偷在线传媒,亚洲精品在线,久久无码精品九号,欧洲av人人爽爽,亚洲精品蜜桃久久久久久,亚洲精品久久午夜无码专区电影,辽宁少妇高潮45分钟,少妇高清性色生活片,无码八少妇久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pPW121
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pPW121
pPW121
規(guī)格:
貨期:
編號:B234314
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pPW121
商品貨號 B234314
Designations pPW121
Depositors F Puta
Biosafety Level 1
Vector Information
Size (kb): 4.3860001564025880
Vector: pPW121 (plasmid)
Promoters: Promoter lambda PR
Construction: pUN121
Marker(s):ampR,tetR
Construct size (kb): 4.386000156402588
Features: marker(s): ampR
marker(s): tetR
operator: lac
promoter: lambda PR
replicon: pMB1
repressor gene: cI
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; BamHI--4.3.
Postive selection cloning vector which confers tetR (recommended concentration 10 ug/ml) only when an insert has been successfully cloned into the cI repressor gene.
Plasmid was derived from pUN121 by introduction of a BglII site into the cI gene. Plasmid pUN121 was derived from pTR262 by addition of EcoRI and SmaI/XmaI sites in the cI gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Puta F, Wambutt R. Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments. Folia Microbiol. 37: 193-198, 1992. PubMed: 1505881

Nilsson B, et al. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11: 8019-8030, 1983. PubMed: 6316281

Frantisek Puta, personal communication

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
永昌县| 古蔺县| 大同县| 常德市| 曲麻莱县| 夏津县| 巨野县| 景德镇市| 望奎县| 浏阳市| 治多县| 偃师市| 东宁县| 姚安县| 桐城市| 洛南县| 麻城市| 金堂县| 建德市| 西峡县| 连山| 城固县| 沙雅县| 鸡西市| 合阳县| 左云县| 遂昌县| 乐至县| 海丰县| 上饶县| 个旧市| 黄浦区| 安西县| 芮城县| 大邑县| 监利县| 临桂县| 定结县| 德兴市| 洞口县| 从化市|