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pCLL4
pCLL4
規(guī)格:
貨期:
編號:B237921
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCLL4
商品貨號 B237921
Designations pCLL4
Depositors American Cyanamid Co., JS Feitelson, American Cyanamid Co.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 10.36
Vector: pCLL4 (plasmid)
Construction: pAkij1, pIJ39
Marker(s):ampR,tsrR
Construct size (kb): 10.36
Features: marker(s): ampR
marker(s): tsrR
replicon: pAkij1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.0, 2.1; PstI--10.5; KpnI--10.5.
Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.
The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.
Recommendation for verification: HindIII--8.37, 1.99; PstI--10.36; KpnI--9.47, 0.89
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

Shipped freeze-dried
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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